Lise Roy is an actor for theatre, cinema and television. Teaching has always occupied a great part of her personal and professional life. After completing a master's degree in creative research, she is now a professor of acting at the École supérieure de théâtre at the Université du Québec à Montréal (UQAM).

On stage, she was noticed for her performance as Camille Claudel in the show Camille C and for her powerful portrayal of Elisabeth the 1st in Marie Stuart (directed by Alexandre Marine). Other shows mark her presence in the theatre industry, including Top Girls (Martine Beaulne), La Casa azul and La Géométrie des miracles (Robert Lepage), Le Bruit des os qui craquent (Gervais Gaudreault), Tom à la ferme (Claude Poissant), Les Bonnes (Marc Béland), Les robots fonts-ils l'amour? (Angela Konrad), the more recent success La meute (Marc Beaupré) and in the play Golgotha picnic (Angela Konrad). In 2014, she produced the theatrical creation Je ne suis jamais en retard; a show involving nearly twenty women: writers, designers and actresses. Over the years, she took part in numerous tours in Quebec with the TPQ, in Europe and the United States with Ex Machina, and in France and Mexico with Carrousel Theatre.

She is in several television series, notably Toute la vérité,  Les invincibles, Nouvelle adresse, Victor Lessard, Les invisibles, Bête noire, La faille 3, Une affaire criminelle and Les bombes. She was seen on the big screen in Les Invasions Barbares, Le Secret de ma mère, Peau Blanche, À l’origine d’un cri and Tom à la ferme, for which she won the award for "Best Supporting Actress in a Canadian Film" at the Vancouver Film Critics Circle's and a nomination for "Best Actress " at the Jutra Awards in 2015. She also received a Gemeni Award for "Best Actress in a Supporting Role" for her work in The Boys of St. Vincent, and a nomination for her portrayal of Miss Lafleur in Children of My Heart.