Lise Roy is a theater, television and film actress. Teaching has been an important part of her career path, and after completing a master's degree in creative research, she taught for several years at the École supérieure de théâtre de l'Université du Québec à Montréal (UQAM).

In the theater, she recently took part in a reading of Que notre joie demeure (Angela Konrad). She has also appeared as Camille Claudel in Camille C and as a powerful Elizabeth I in Marie Stuart (Alexandre Marine). Her other theater credits include Top Girls (Martine Beaulne), La Casa azul and La Géométrie des miracles (Robert Lepage), Le Bruit des os qui craquent (Gervais Gaudreault), Tom à la ferme (Claude Poissant), Les Bonnes (Marc Béland), Les robots fonts-ils l'amour? and Golgotha picnic (Angela Konrad), as well as the most recent success, La meute (Marc Beaupré). In 2014, she produced the theatrical creation Je ne suis jamais en retard, involving nearly twenty women authors, designers and actors. Over the years, she has toured extensively in Quebec with TPQ, in Europe and the U.S. with Ex Machina, and in France and Mexico with Théâtre le Carrousel.

Her film credits include Lucy, Grizzli, Sophie directed by Anne Émond, Les Invasions Barbares, Le Secret de ma mère, Peau Blanche, À l'origine d'un cri and Tom à la ferme, for which she won the Vancouver Film Critics Circle award for “Best Supporting Actress in a Canadian Film” and a nomination for Best Actress at the 2015 Jutra Awards. She also received the Gemini Award for Best Supporting Actress for The Boys of St-Vincent, as well as a nomination for her portrayal of Miss Lafleur in Children of my heart. She has also appeared in several TV series, including Toute la vérité, Les invincibles, Nouvelle adresse, Victor Lessard, Les invisibles, Bête noire, La faille 3, Une affaire criminelle and Les bombes.